Heterogeneity in tissue ferritins displayed by gel electrofocusing

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Heterogeneity in tissue ferritins displayed by gel electrofocusing.

1. Horse spleen ferritin and human liver ferritin were examined by gel electrofocusing under conditions that demonstrated equilibrium focusing. Both ferritins were resolved into multiple isoferritins. Both families of isoferritins were separable from one another. 2. Horse spleen ferritin was also resolved into five components by ion-exchange chromatography on DEAE-Sephadex A-50. Each of the maj...

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Separation of the proteins of cerebrospinal fluid using gel electrofocusing followed by electrophoresis.

The technique previously applied to serum can, with minor modifications, be applied to cerebrospinal fluid. Good protein patterns have been obtained and similarities as well as differences have been demonstrated between cerebrospinal fluid and serum. The patterns obtained should prove useful in neurological diagnosis.

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Alterations in tissue ferritins in iron storage disorders.

Purified tissue ferritins isolated from Bantu subjects with gross haemosiderosis, from a patient with idiopathic haemochromatosis (HC) treated by phlebotomy, and from rats with experimental iron overload were studied in order to determine the significance of the abnormality previously demonstrated in tissue isoferritins in patients with IHC. The isoferrin profile of the tissues from the Bantu s...

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The size and shape of heart and muscle ferritins analyzed by sedimentation, gel filtration, and electrophoresis.

We have compared the size and shape of ferritins from human heart, rat heart, and skeletal muscle with those of rat liver and horse spleen ferritins, using sedimentation and gel filtration techniques. The electrophoretically "fast" form of heart ferritin was partially separated from both the "slow" heart form and from rat liver ferritin by gel filtration (Stokes radii 72 A versus 69 and 68.5 A)...

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Dosimetric Study of Tissue Heterogeneity Correction for Breast Conformal Radiotherapy

Introduction: Heterogeneity correction is an important parameter in dose calculation for cancer patients where it may be cause inaccuracy in dose calculation as a result of different densities of patients. This study studied the impact of dose calculation of breast cancer patients with and without heterogeneity correction. Material and Methods: Twenty breast cancer patients were treated with Th...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1974

ISSN: 0264-6021

DOI: 10.1042/bj1410627